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關于ibiBoostTM Adeno Adenovirus Transduction Enhancer
易必迪 ibidi 腺病毒轉導強化劑ibiBoostTM Adeno Adenovirus Transduction Enhancer
貨 號：50301
A support reagent for enhancing the efficiency of
viral gene transfer into difficult to transduce
mammalian and rodent cells
Efficient, due to 20- to 50-fold enhanced adenovirus
transduction
A non-toxic, peptide-based solution
Gives access to nearly all cell types
ibiBoostTM Adeno Adenovirus Transduction Enhancer Applications:
Adenovirus transduction
Please note that you will be working with samples containing an
infectious virus. Follow the recommended NIH guidelines for all
materials containing BSL–2 organisms.
ibiBoostTM Adeno Adenovirus Transduction Enhancer Technical Features:
Gives access to cells that express little CAR (Coxsackie
Adenovirus Receptor)
to improve the uptake of adenovirus vectors (e.g., rAV-LifeAct Adenoviral Vectors ) into cells and cell lines that are normally
difficult to transduce. ibiBoost reagent is unique, because it is based
on an adenovirus binding peptide. Due to this factor, ibiBoost changes
the adenovirus surface in a way that the uptake of the adenovirus is
improved for all permissive cell types, and it allows transduction of
cell types previously not accessible to adenovirus transduction. Unlike
chemical transduction enhancers, ibiBoost has no toxic effects. The use
of ibiBoost is especially recommended if high ”Multiplicities of
Infection“ (MOI), exceeding 500, have to be applied to a particular cell type.
ibiBoostTM Adeno Adenovirus Transduction Enhancer Specifications:
|
Volume |
50 μl |
|
Amount |
For 50 transductions à 2 × 107 IU |
|
Storage |
4°C |
ibiBoost? Adeno Adenovirus Transduction
Enhancer significantly enhanced GFP
expression in rodent NIH-3T3 cells
Enhancing Transduction Efficiency
I: Genetic manipulation of target cells increases the CAR expression
on the cell surface.
II: Genetic manipulation of the adenovirus binding domain addresses
the non-CAR surface receptors of the target cell.
III: The ibiBoost Adeno Adenovirus Transduction Enhancer bridges the
adenovirus surface to the cell membrane, thereby enhancing the gene
expression by 20- to 50-fold into cell types that lack the primary adenovirus receptor CAR.
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